Bin2 phosphorylation mutant

Author: ogiy

August undefined, 2024

WebBIN2-mediated phosphorylation of ARF7 and ARF19 suppresses their interaction with AUX/IAAs, and subsequently enhances the transcriptional activity to their target genes … WebThus, BIN2 is a good candidate to regulate PIF4 levels, as inactivation of this kinase by BL may result in the fastest-migrating form of this factor seen in BL-treated seedlings ( Fig. 1C ). A direct interaction of the full-length BIN2 protein and PIF4 was actually observed in yeast two-hybrid assays ( Fig. 2A ).

A secreted peptide acts on BIN2-mediated phosphorylation of

WebOct 6, 2024 · In order to test if they are BIN2 kinase phosphorylation sites, we mutated the two sites from serine to alanine (phosphorylation null, designated as BES1 S2A) and tested phosphorylation by BIN2 in ... WebMar 30, 2024 · The BIN2 gain-of-function mutant bin2-1 displays an enhanced photomorphogenic phenotype in both far-red and red lights. phyA-enhanced accumulation of BIN2 promotes the phosphorylation of BES1 in far-red light. BIN2 acts genetically downstream from PHYA to regulate photomorphogenesis under far-red light. green thyme mattress

Integrated omics networks reveal the temporal signaling events of ...

WebNov 11, 2024 · e, Western blot with the custom-made p-BIN2 antibody on BIN2-WT or BIN2-AA (T423A and S424A non-phosphorylable mutant)-expressing A2780 cells showed that the p-BIN2 antibody detected an expected ... WebMar 21, 2024 · GeneCards Summary for BIN2 Gene. BIN2 (Bridging Integrator 2) is a Protein Coding gene. Among its related pathways are Innate Immune System and … WebBIN2 is involved in a wide range of developmental processes through phosphorylating a multitude of substrates, including many TFs (Youn and Kim, 2015 ). To test this hypothesis that RD26 is phosphorylated by … green tiana clip art

BR-dependent phosphorylation modulates PIF4 transcriptional activity ...

MYBL2 is a substrate of GSK3-like kinase BIN2 and acts as …

WebApr 6, 2015 · AtSK21/BIN2 phosphorylation of ARF2 alleviates DNA binding and repressor activity of ARF2, ... Notably, mutant bin2-1 protein accumulates in greater quantities than wild-type AtSK21/BIN2 in plant cells (Peng et al., 2008). The BR biosynthetic inhibitor BRZ induces the accumulation of AtSK21/BIN2 in plant cells. http://genesdev.cshlp.org/content/28/15/1681.long green tick air force 1WebAug 13, 2024 · Phosphorylation mediated by protein kinases has been extensively studied, as it is one of the most important and ubiquitous posttranslational modifications. Protein kinase-mediated phosphorylation modulates many cellular processes, including the activities of enzymes, signal transduction, transcription, and metabolism. green tick and red cross

"To further identify the mechanism by which HY5 regulated BIN2 in the light to destabilize BZR1, we first checked whether HY5 regulated the mRNA and/or protein levels of BIN2. As shown in Supplementary Fig. 5, both the mRNA and protein levels of BIN2 were similar in hy5 and WT, suggesting that HY5 does not … See more To investigate the cross-talk between light and BR signaling, we performed yeast two-hybrid analyses and found that BIN2, the key negative … See more Next, we investigated whether HY5 could genetically interact with BIN2 by crossing hy5 into bin2-1, a gain-of-function mutant of BIN228. We found that the hypocotyls of double mutant … See more To understand which amino acid residues of HY5 were important for its interaction with BIN2, we screened BIN2-interacting sites in the HY5 LZ region. As shown in Fig. 5a, changing HY5 Leu137 (L137) to Asp (named HY5-M) … See more Previous reports have suggested that HY5 and BIN2 have similar effects on BZR1 protein accumulation20,27. To gain further insight into their … See more " - Bin2 phosphorylation mutant

Bin2 phosphorylation mutant

BR-dependent phosphorylation modulates PIF4 transcriptional …

WebThe BIN2 gain-of-function mutant bin2-1 displays an enhanced photomorphogenic phenotype in both far-red and red lights. phyA-enhanced accumulation of BIN2 promotes … WebSep 6, 2009 · GST–BIN2 M115A is a BIN2 kinase-inactive mutant. (e) GST–BIN2 or GST–bin2-1 was first treated with BSU1–YFP immunoprecipitated from BR-treated (+ BL; 0.25 μM for 30 min) or untreated 35S ...

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WebBIN2 phosphorylation of RD26 is required for RD26 transcriptional activation on drought-responsive genes. RD26 overexpression suppressed the brassinazole (BRZ) insensitivity of BIN2 triple mutant bin2 bil1 bil2, … WebNov 9, 2024 · GSK3-like Kinases Negatively Regulate Salt Stress Tolerance. To determine if GSK3-like kinases function in the plant salt stress response, we assessed the salt sensitivity of a BIN2, BIL1, and BIL2 triple loss-of-function mutant (bin2-3bil1bil2, abbreviated 3b) (Yan et al., 2009).Seedlings of the 3b mutant had a significantly shorter primary root …

WebJan 10, 2024 · Bottom schematic shows BASL polarization requires MAPK-mediate protein phosphorylation and the plasma membrane-associated BRX proteins. Unknown regulators (orange) are anticipated to mediate BASL polarization. ... Genotyping results showed that these mutant plants are ... GSK3-like BIN2 kinases 12 and the BSL phosphatases 13, … WebBIN2 (brassinosteroid-insensitive 2) kinase, a GSK3-like kinase functioning as a negative regulator in BR signaling, directly phosphorylated RAPTOR1B (regulatory-associated protein of TOR 1B), a substrate-recruiting subunit in the TOR complex, at a conserved serine residue within a typical BIN2 phosphorylation motif.

WebIt was shown that the BIN2-catalyzed phosphorylation of BSK3 promotes the interactions of BSK3 with the BR receptor BRI1 (Brassinosteroid-Insensitive1) ... elimination of GSK3-like kinases resulted in an increased ratio of SE/CC whereas overexpression of a gain-of-function mutant variant bin2-1 slightly decreased the SE/CC ratio, ... WebMar 30, 2024 · The BIN2 gain-of-function mutant bin2-1 displays an enhanced photomorphogenic phenotype in both far-red and red lights. phyA-enhanced …

WebJan 30, 2024 · Importantly, the BIN2 phosphorylation of Thr280 residue restricts the function of CO in promoting flowering through affecting its DNA-binding activity. Moreover, we …

WebBIN2 integrates multiple signaling pathways by phosphorylating a range of proteins including the components in light signaling. For example, PIF3, PIF4, and PIF5 can be phosphorylated by BIN2 and then degraded via the 26S proteasome pathway to control transcription and hypocotyl growth ( Bernardo-García et al., 2014; Ling et al., 2024 ). green tick and red cross in excelWebApr 20, 2024 · In this study, we show that the GSK3 kinase BIN2 physically interacts with CO and the gain-of-function mutant bin2-1 displays late flowering phenotype through down-regulation of FT transcription. Genetic analyses show that BIN2 genetically acts upstream of CO in regulating flowering time. green tiara dishesWebJul 22, 2009 · Plant Materials and Growth Conditions. Arabidopsis thaliana ecotype Columbia was used as WT control. BR-deficient mutant det2-1 and BR-insensitive mutant bin2-1 were obtained from Dr. Zhiyong Wang and Dr. Jun Zhao. WT and det2-1 seeds were surface-sterilized in 10% sodium hypochlorite and rinsed five times in sterile water. The … green tiara sandwich glassWebNov 14, 2024 · In mature MMCs or meristemoids, BIN2 is expressed, is upregulated and initiates BASL polarization redundantly with the MAPK module. The BIN2–POLAR–BASL … green tick animationWebDa Som Lee1,2 iD Tae Hyeon An2,3 iD Hyunmi Kim2,3 iD Eunsun Jung4 iD Gyeonghun Kim5 iD Seung Yeon Oh6 iD Jun Seok Kim7 iD Hye Jin Chun8 iD Jaeeun Jung9 iD Eun-Woo Lee2,3 iD Baek-Soo Han3,10 iD Dai Hoon Han11 iD Yong-Ho Lee8,12 iD Tae-Su Han3,4 iD Keun Hur13 iD Chul-Ho Lee3,14 iD Dae-Soo Kim9 iD Won Kon Kim2,3 iD Jun … greenthusiastsWebAug 1, 2024 · BR-INSENSTIVE 2 (BIN2), a crucial negative regulator of brassinosteroid (BR) signaling, is induced by ABA and phosphorylates SnRK2s to enhance ABA signaling [5]. PP2Cs, such as ABI1 and ABI2, function as negative regulators of both BIN2 and SnRK2s, and thereby form a negative feedback regulatory loop in ABA signaling [12]. green tick and red xWebJun 6, 2024 · Considering the BIN2 inhibitor bikinin greatly increased SA-induced gene expression (Figure 1 D–1F and Supplemental Figure 1 B–1D), we examined the functional involvement of BIN2 in SA signaling.The bin2-1 gain-of-function mutant displayed reduced sensitivity to SA in a bacterial growth assay (Figure 2 B). Similarly, the inhibitory effect of … fnd sinaloa