Bodipy fl-pepstatin a

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WebAug 14, 2024 · For labeling active Cathepsin D by Bodipy FL-pepstatin A, live neurons were incubated with 1 μM Bodipy-FL-pepstatin A in Neurobasal medium for 1 h at 37 °C, followed by fixation and immunostaining with antibodies against MAP2 and LAMP-1. For labeling active Cathepsin B by the cresyl violet fluorogenic substrate CV-(Arg-Arg)2 … WebIn vitro, BODIPY FL-pepstatin A inhibits cathepsin D activity with an IC 50 of 10 nM. The nature of its binding to cathepsin D was further characterized using a fluorescence …

Antitumor activity of metal-chelating compound Dp44mT is ... - PubMed

WebMar 16, 2000 · BODIPY FL–pepstatin A exhibits potent inhibitory effect on cathepsin D activity. Cathepsin D (50 nM) was incubated with various concentration of inhibitors in … WebThermo Fisher bodipy fl pepstatin a Bodipy Fl Pepstatin A, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. … department of buildings now

Probing the cathepsin D using a BODIPY FL-pepstatin A: …

WebNov 20, 2024 · LysoTracker Red and BODIPY FL-pepstatin A labeling. Hela cells were cultured with 50 n m LysoTracker Red for 30 min at 37 °C. HEK cells grown on LabTek chambers were incubated with 1 μ m BODIPY FL-pepstatin A for 1 h at 37 °C. After washing with PBS to remove the excess probes, the cells were visualized by confocal … WebSep 1, 2011 · This targeting was confirmed with pepstatin A-BODIPY FL, which showed redistribution of cathepsin D to the cytosol with ensuing cleavage of the proapoptotic BH3 protein Bid. Redox activity of Cu[Dp44mT] caused cellular depletion of glutathione, and lysosomal damage was prevented by cotreatment with the glutathione precursor N … WebMar 16, 2024 · Cathepsin D activation in lysosomes was measured using Bodipy FL-pepstatin A. An increase in cathepsin D was only observed at the high concentration of dimebon and chloropromazine at 4 hrs ( Fig 2C ), however by 24 hrs, all tested drugs increased cathepsin D activity at multiple concentrations ( Fig 2D) Download: PPT … department of buildings nj

Antitumor Activity of Metal-Chelating Compound Dp44mT Is …

WebProduct Overview. Recommendations. Documents. For the study of cathepsin D trafficking in cells by fluorescence microscopy, try our BODIPY FL pepstatin A. This green … TC-FlAsH II In-Cell Tetracysteine Tag Detection Kit contains an expression tag … For the study of cathepsin D trafficking in cells by fluorescence microscopy, try our … TaqMan Real-Time PCR Assays. Antibodies. Oligos, Primers & Probes Support How can we help? Choose an option. Contact Us WebPepstatin A, BODIPY FL conjugate is a green-fluorescent probe for investigation of cathepsin D tracking in cells. Pepstatin A selectively binds to cathepsin D at pH 4.5. … fha requirement for townhouse loan reserveWebBodipy Fl Pepstatin A, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, … fha requirements for gutters and downspouts

"WebAug 30, 2011 · This targeting was confirmed with pepstatin A–BODIPY FL, which showed redistribution of cathepsin D to the cytosol with ensuing cleavage of the proapoptotic BH3 protein Bid. Redox activity of Cu[Dp44mT] caused cellular depletion of glutathione, and lysosomal damage was prevented by cotreatment with the glutathione precursor N … " - Bodipy fl-pepstatin a

Bodipy fl-pepstatin a

Pepstatin A, BODIPY FL Conjugate - BOC Sciences

WebThe BODIPY FL-pepstatin A is a potent inhibitor for cathepsin D. It inhibits the protease activity of cathepsin D with an IC 50 ~50 nM; 2. The binding of BODIPY FL-pepstatin A … WebDescription. BODIPY™ FL pepstatin A, a green-fluorescent probe, which inhibits cathepsin D in vitro (IC50 approx. 50nM), targets cathepsin D within lysosomes of live cells. Initial …

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WebInvitrogen™ Pepstatin A, BODIPY™ FL Conjugate For the study of cathepsin D trafficking in cells by fluorescence microscopy Brand: Invitrogen™ P12271 Additional Details : … WebInvitrogen™ Pepstatin A, BODIPY™ FL Conjugate For the study of cathepsin D trafficking in cells by fluorescence microscopy Brand: Invitrogen™ P12271 Additional Details : Weight : 0.00525kg View more versions of this product Product Code. 11589196 343.00 € / 25µg Add to basket Description Specifications Documents

WebIn vitro, BODIPY FL-pepstatin A inhibits cathepsin D activity with an IC50 of 10 nM. The nature of its binding to cathepsin D was further characterized using a fluorescence … WebApr 1, 2000 · BODIPY-FL pepstatin A exhibited selective, active site binding to Na-APR-1 wt as evidenced by its ability to inhibit aspartic protease activity and compete with unlabelled pepstatin A for binding ...

WebOther suitable methodologies rely on the use of fluorescently labelled inhibitors of lysosomal hydrolases such as Bodipy FL-Pepstatin A (Invitrogen, P12271), a … Web(E) BODIPY FL-pepstatin A (Green) staining representing the excess CTSD lysosomal uptake in LKO hepatocytes with the accompanying histograms showing quantitation. n > 20 cells from each group analyzed in C-E. Bars represent mean ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001, using the Student’s t test. Scale: 20 μm Display full size

WebMay 13, 2024 · (E) Differential expression of active CTSD as indicated by live staining with BODIPY-FL-pepstatin A in WT, casp9 KO, and rescued casp9 KO MEF. Scale bar: 60 μm. (F) Quantification of live staining with BODIPY-FL-pepstatin A is shown in D. The data are means±SEM of approximately 50 cells in each of 3 independent experiments. *** = p < …

WebMar 1, 2006 · Pepstatin A, an Aspartic Proteinase Inhibitor, Suppresses RANKL-Induced Osteoclast Differentiation The Journal of Biochemistry Oxford Academic Abstract. Pepstatin A is well known to be an inhibitor of aspartic proteinases such as pepsin, cathepsins D and E. Except for its role as a proteinase inhibitor department of buildings staten island nyWebJan 28, 2024 · To determine whether enlarged Cath D positive compartments in the perinuclear region were autolysosomes, we transduced i 3 Neurons with a lentivirus encoding the autophagic marker, LC3 fused to Halo-Tag (Halo-LC3) and loaded the cells with BODIPY-FL-pepstatin A, a fluorescent probe that binds specifically to active Cath … fha requirements for deck railingWeb(A) Representative images of WT and sAC KO MEFs stained with BODIPY-FL–Pepstatin A. (B) BODIPY-FL–Pepstatin A intensity was quantified using MetaMorph in multiple cells from three independent... department of building violation checkWebFig. 9. Saturation binding analysis for BODIPY FL GTP γ S binding to G αo in a 96-well plate format. G αo (20 n M) was incubated with the indicated concentrations of BODIPY FL GTP γ S for 20 min at room temperature (24°). Excitation was at 485 nm, while emission was measured with a photomultiplier tube behind a 530 nm long-pass filter. fha requirements for gfci outletsWebOct 28, 2013 · Probing the cathepsin D using a BODIPY FL-pepstatin A: applications in fluorescence polarization and microscopy. J. Biochem. Biophys. Methods (2000) ... Pepstatin A was also shown to inhibit HUVEC chemotaxis and tube formation, at least partly through its inhibition of cathepsin D activation of MMP-9 [61]. In vivo, pretreatment … fha requirements for in ground poolWeb开馆时间：周一至周日7:00-22:30 周五 7:00-12:00; 我的图书馆 department of buildings town of hempsteadWebJul 3, 2024 · The intensity of BODIPY FL (BP) fluorescence after cells were incubated with BODIPY FL-Pepstatin A for 30 min revealed a 45% reduction in the level of CSTD activity in the lysosomes of intact DS fibroblasts compared with that in 2N control cells ( Fig. 3 A, B; t(85) = 12.14, p < 0.0001; unpaired t test). department of bureau and labor statistics