WebThe Ion Library TaqMan ™ Quantitation Kit contains reagents for quantitative real-time PCR (qPCR) for the quantification of unamplified and amplified libraries prepared using … Web14 jul. 2024 · Libraries were purified using Ampure XP beads before quantitation by qPCR with the Ion Library TaqMan™ quantitation Kit (Cat: 4468802; Thermo Fisher Scientific, UK). Libraries were normalised to 70pM before pooling and templating onto an Ion 540™ Chip (Cat: A22765, Thermo Fisher Scientific, UK) using an Ion Chef™ (Cat: 4484177, …
PRO029.5 Protocol, FusionPlex® Reagents for Ion Torrent
Web1 apr. 2024 · Libraries were barcoded using Ion Xpress Barcode 1-16 Kit (4471250, Thermo-Fisher Scientific). Libraries were quantified using the Ion TaqMan Quantitation Kit (4468802, Thermo-Fisher Scientific) in Fast Mode using the 7900HT (Thermo-Fisher Scientific). Libraries were diluted to 75pM for sequencing. Web(PCR) using an Ion Library TaqMan Quantitation Kit (Thermo Fisher Scientific, Inc., cat. no. 4468802). Emulsion PCR and sample enrichment were performed using the Ion PI HI-Q Template OT2 200 Kit v2 (Thermo Fisher Scientific, Inc., cat. no. A26434), according to the manufacturer’s instructions. Isolation of the templates on Ion Sphere diamond gradings explained
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Webtions of the libraries were determined by qPCR using an Ion Library Taqman Quantitation Kit (Termofisher, Cat. No. 4468802). Emulsion PCR and sample enrichment were performed using the Ion PI HI-Q Template OT2 200 Kit v2 (Termofisher, Cat.no.A26434), according to the manufacturer’s instructions. Isolation of templated on the WebAn easy-to-use, flexible solution for the accurate quantitation of library input for the Ion Torrent semiconductor sequencing workflow. Brand: TaqMan™ Ion Torrent™ 4468802. … Web4468802 Ion Library TaqMan™ Quantitation Kit Packaging Lot: 01182811 Date of Manufacture: 03.11.2024 (DD.MM.YYYY) QUALITY CONTROL ISO CERTIFICATION … circular saw invented by a woman